Photosynthetica 2021, 59(4):615-624 | DOI: 10.32615/ps.2021.047

Mitochondrial alternative oxidase pathway acts as an electron sink during photosynthetic induction in Rumex K-1 leaves

D.D. CHENG1, L.T. ZHANG2
1 College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong, China
2 Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China

Chloroplast PSII photochemical efficiency is upregulated more rapidly than CO2 assimilation during photosynthesis induction, suggesting the existence of other electron sinks than that of CO2 assimilation. We hypothesized that the mitochondrial alternative oxidase (AOX) pathway could be such a sink. Inhibition of the AOX restricted light activation of the malate-oxaloacetate shuttle and caused an excessive reduction of PSI acceptor side and substantial accumulation of QA-, hindering the photosynthetic linear electron transport rate (ETR) and leading to an imbalance between light energy absorption and exploitation during photosynthetic induction. ETR limitation also restricted the formation of thylakoid pH gradient, evidenced by a decreased de-epoxidation of the xanthophyll cycle, thus preventing nonphotochemical quenching. Delayed CO2 assimilation due to thylakoid pH gradient restriction was partially reversed by exogenous ATP application. The AOX pathway acts as a photosynthetic electron sink, protecting the photosynthetic apparatus against photoinhibition and accelerating the induction of CO2 assimilation during photosynthetic induction in Rumex K-1 leaves.

Additional key words: alternative oxidase; photodamage; photosystem II photochemical efficiency; photosynthetic induction; Rumex K-1.

Received: March 10, 2021; Revised: September 28, 2021; Accepted: October 5, 2021; Prepublished online: November 23, 2021; Published: December 17, 2021  Show citation

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CHENG, D.D., & ZHANG, L.T. (2021). Mitochondrial alternative oxidase pathway acts as an electron sink during photosynthetic induction in Rumex K-1 leaves. Photosynthetica, 59(4), 615-624. doi: 10.32615/ps.2021.047
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