Photosynthetica 2021, 59(4):633-639 | DOI: 10.32615/ps.2021.053

Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease

M. ADAMIEC1, M. SZOMEK2, E. GABAŁA3, J. DOBROGOJSKI4, L. MISZTAL1, R. LUCIŃSKI1
1 Adam Mickiewicz University, Faculty of Biology, Institute of Experimental Biology, Department of Plant Physiology, Umultowska 89, 61-614 Poznań, Poland
2 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, M 5230 Odense, Denmark
3 Institute of Plant Protection, National Research Institute, Węgorka 20, 60-318 Poznań, Poland
4 Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11, 60-637 Poznań, Poland

EGY1 (ethylene-dependent gravitropism-deficient and yellow-green 1) is an intramembrane metalloprotease located in chloroplasts, involved in many diverse processes including chloroplast development, chlorophyll biosynthesis, and the ethylene-dependent gravitropic response. Plants deprived of this protease display pleiotropic effects such as the yellow-green early senescence phenotype and a poorly developed thylakoid system membrane in the mature chloroplasts. We applied the GC/MS technique to analyze the changes in fatty acid composition in two egy1 mutant lines. We used DAPI staining and transmission electron microscopy methods to establish the number of nucleoids and the amount of chloroplast DNA. Our results indicated that the lack of EGY1 protease led to a dramatic overaccumulation and a dramatic decrease in the content of linolenic acid C18:3 and hexadecatrienoic acid C16:3, respectively. The amount of chloroplast DNA and the number of nucleoids were severely reduced in egy1 mutant lines. Similarly, a reduced correlation between DAPI and autofluorescence signal was observed, which may indicate some perturbations in nucleoid anchoring.

Additional key words: Arabidopsis thaliana; chloroplasts; EGY1; fatty acids; nucleoids.

Received: August 4, 2021; Revised: October 21, 2021; Accepted: November 2, 2021; Prepublished online: November 25, 2021; Published: December 17, 2021  Show citation

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ADAMIEC, M., SZOMEK, M., GABAŁA, E., DOBROGOJSKI, J., MISZTAL, L., & LUCIŃSKI, R. (2021). Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease. Photosynthetica, 59(4), 633-639. doi: 10.32615/ps.2021.053
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