Photosynthetica 2022, 60(4):539-548 | DOI: 10.32615/ps.2022.050

Photosynthetic responses of Eulophia dentata, Bletilla formosana, and Saccharum spontaneum under various photosynthetic photon flux density conditions

Y.-H. LAI1, †, K.-H. LIN2, †, C.-P. LIU1, T.-S. LIAO1, M.-Y. HUANG3, C.-W. WANG4, C.-I. CHEN5
1 Department of Forestry, National Chung Hsing University, 40227 Taichung, Taiwan
2 Department of Horticulture and Biotechnology, Chinese Culture University, 11114 Taipei, Taiwan
3 Department of Life Sciences and Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, 40227 Taichung, Taiwan
4 Endemic Species Research Institute, 552203 Nantou, Taiwan
5 Department of Forestry, National Pingtung University of Science and Technology, 91201 Pingtung, Taiwan

This study aimed to determine the photosynthetic performance and differences in chlorophyll fluorescence (ChlF) parameters between Eulophia dentata and its companion species Bletilla formosana and Saccharum spontaneum when subjected to different photosynthetic photon flux density (PPFDs). Leaf surfaces were then illuminated with 50, 100 (low PPFDs), 300, 500, 800 (moderate PPFDs); 1,000; 1,500; and 2,000 (high PPFDs) μmol m-2.s-1, and the ChlF parameters were measured during the whole process. Increasing nonphotochemical quenching of ChlF and decreasing potential quantum efficiency of PSII, actual quantum efficiency of PSII, and quantum efficiency ratio of PSII in dark recovery from 0-60 min were observed in all leaves. A significant and negative relationship was detected between energy-dependent quenching (qE) and photoinhibition percent in three species under specific PPFD conditions, whereas a significant and positive relationship was detected between photoinhibitory quenching (qI) and photoinhibition percent. The qE and qI can be easily measured in the field and provide useful ecological indexes for E. dentata species restoration, habitat creation, and monitoring.

Additional key words: Bletilla; chlorophyll fluorescence; Eulophia; light intensity; photoinhibition; Saccharum.

Received: May 1, 2022; Revised: October 21, 2022; Accepted: November 22, 2022; Prepublished online: December 16, 2022; Published: December 21, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
LAI, Y.-H., LIN, K.-H., LIU, C.-P., LIAO, T.-S., HUANG, M.-Y., WANG, C.-W., & CHEN, C.-I. (2022). Photosynthetic responses of Eulophia dentata, Bletilla formosana, and Saccharum spontaneum under various photosynthetic photon flux density conditions. Photosynthetica, 60(4), 539-548. doi: 10.32615/ps.2022.050
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileLai_2920_supplement.docx

    File size: 3.08 MB

    References

    1. Adams III W.W., Zarter C.R., Ebbert V., Demmig-Adams V.: Photoprotective strategies of overwintering evergreens. - BioScience 54: 41-49, 2004. Go to original source...
    2. Björkman O., Demmig-Adams B.: Regulation of photosynthetic light energy capture, conversion, and dissipation in leaves of higher plants. - In: Schulze E.D., Caldwell M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 17-47. Springer, Berlin-Heidelberg 1995. Go to original source...
    3. Bussotti F., Gerosa G., Digrado A., Pollastrini M.: Selection of chlorophyll fluorescence parameters as indicators of photosynthetic efficiency in large scale plant ecological studies. - Ecol. Indic. 108: 105686, 2020. Go to original source...
    4. Chang L.H., Liu C.H., Li. C.Y.: [Asymbiotic germination and plant regeneration of Eulophia dentate Ames, a critically endangered plant in Taiwan.] - Taiwan J. Biodivers. 16: 241-251, 2014.
    5. Colom M.R., Pini Prato E., Giannini R.: Chlorophyll fluorescence and photosynthetic response to light in 1-year-old needles during spring and early summer in Pinus leucodermis. - Trees-Struct. Funct. 17: 207-210, 2003. Go to original source...
    6. Demmig-Adams B., Adams III W.W., Barker D.H. et al.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. - Physiol. Plantarum 98: 253-264, 1996. Go to original source...
    7. Demmig-Adams B., Adams III W.W., Mattoo A.K.: Photoprotection, Photoinhibition, Gene Regulation, and Environment. Pp. 382. Springer, Dordrech 2006. Go to original source...
    8. Demmig-Adams B., Stewart J.J., López-Pozo M. et al.: Zeaxanthin, a molecule for photoprotection in many different environments. - Molecules 25: 5825, 2020. Go to original source...
    9. Dewir Y.H., El-Mahrouk M.E.S., Al-Shmgani H.S. et al.: Photosynthetic and biochemical characterization of in vitro-derived African violet (Saintpaulia ionantha H. Wendl) plants to ex vitro conditions. - J. Plant Interact. 10: 101-108, 2015. Go to original source...
    10. Editorial Committee of the Red List of Taiwan Plants: The Red List of Vascular Plants of Taiwan, 2017. Pp. 198. Endemic Species Research Institute, Forestry Bureau, Council of Agriculture, Executive Yuan and Taiwan Society of Plant Systematics, 2017.
    11. Giudici G.N.M.: Photoinhibition of primary photosynthetic processes in hydrated Polytrichum commune: analysis of non-photochemical quenching affecting species resistance. - Czech Polar Rep. 9: 160-169, 2019. Go to original source...
    12. Guidi L., Lo Piccolo E., Landi M.: Chlorophyll fluorescence, photoinhibition and abiotic stress: does it make any difference the fact to be a C3 or C4 species? - Front. Plant Sci. 10: 174, 2019. Go to original source...
    13. Johnson M.P., Ruban A.V.: Restoration of rapidly reversible photoprotective energy dissipation in the absence of PsbS protein by enhanced ΔpH. - J. Biol. Chem. 286: 19973-19981, 2011. Go to original source...
    14. Kalapchieva S., Topalova E., Petkova V.: Morphological, physiological and productivity response in garden pea genotypes during high temperature stress. - Genetika 51: 417-428, 2019. Go to original source...
    15. Ka³u¿ewicz A., B±czek-Kwinta R., Krzesiñski W. et al.: Effect of biostimulants on chlorophyll fluorescence parameters of broccoli (Brassica oleracea var. italica) under drought stress and rewatering. - Acta Sci. Pol.-Hortoru. 17: 97-106, 2018. Go to original source...
    16. Li Z., Wang G., Liu X. et al.: Genome-wide identification and expression profiling of DREB genes in Saccharum spontaneum. - BMC Genomics 22: 456, 2021. Go to original source...
    17. Lin C.W., Hwang T.L., Chen F.A. et al.: Chemical constituents of the rhizomes of Bletilla formosana and their potential anti-inflammatory activity. - J. Nat. Prod. 79: 1911-1921, 2016. Go to original source...
    18. Makarenko M.S., Kozel N.V., Usatov A.V. et al.: A state of PSI and PSII photochemistry of sunflower yellow-green plastome mutant. - J. Biol. Sci. 16: 193-198, 2016. Go to original source...
    19. Malnoë A.: Photoinhibition or photoprotection of photosynthesis? Update on the (newly termed) sustained quenching component qH. - Environ. Exp. Bot. 154: 123-133, 2018.
    20. Maxwell K., Johnson G.N.: Chlorophyll fluorescence - a practical guide. - J. Exp. Bot. 51: 659-668, 2000. Go to original source...
    21. Moya I., Loayza H., López M.L. et al.: Canopy chlorophyll fluorescence applied to stress detection using an easy to build micro-lidar. - Photosynth. Res. 142: 1-15, 2019. Go to original source...
    22. Murchie E.H., Niyogi K.K.: Manipulation of photoprotection to improve plant photosynthesis. - Plant Physiol. 155: 86-92, 2011. Go to original source...
    23. Müller P., Li X.P., Niyogi K.K.: Non-photochemical quenching. A response to excess light energy. - Plant. Physiol. 125: 1558-1566, 2001. Go to original source...
    24. Nilkens M., Kress E., Lambrev P. et al.: Identification of a slowly inducible zeaxanthin-dependent component of non-photochemical quenching of chlorophyll fluorescence generated under steady-state conditions in Arabidopsis. - BBA-Biomembranes 1797: 466-475, 2010. Go to original source...
    25. Orekhova A., Barták M., Casanova-Katny A., Hájek J.: Resistance of Antarctic moss Sanionia uncinata to photoinhibition: chlorophyll fluorescence analysis of samples from the western and eastern coasts of the Antarctic Peninsula. - Plant Biol. 23: 653-663, 2021. Go to original source...
    26. Pandey V.C., Bajpai O., Pandey D.N., Singh N.: Saccharum spontaneum: an underutilized tall grass for revegetation and restoration programs. - Genet. Resour. Crop Evol. 62: 443-450, 2015. Go to original source...
    27. Pandey V.C., Sahu N., Singh D.P.: Physiological profiling of invasive plant species for ecological restoration of fly ash deposits. - Urban For. Urban Gree. 54: 126773, 2020. Go to original source...
    28. Papageorgiou G.C., Govindjee: The non-photochemical quenching of the electronically excited state of chlorophyll a in plants: definitions, timelines, viewpoints, open questions. - In: Demmig-Adams B., Garab G., Adams III W.W., Govindjee (ed.): Non-Photochemical Quenching and Energy Dissipation in Plants, Algae and Cyanobacteria. Pp. 1-44. Springer, Dordrecht 2014. Go to original source...
    29. Pastenes C., Horton P.: Effect of high temperature on photosynthesis in beans. (I. Oxygen evolution and chlorophyll fluorescence) - Plant Physiol. 112: 1245-1251, 1996. Go to original source...
    30. Portela F.C.S., Macieira B.P.B., Zanetti L.V. et al.: How does Cariniana estrellensis respond to different irradiance levels? - J. Forestry Res. 30: 31-44, 2019. Go to original source...
    31. Rosa-Manzano E., Andrade J.L., García-Mendoza E. et al.: Photoprotection related to xanthophyll cycle pigments in epiphytic orchids acclimated at different light micro­environments in two tropical dry forests of the Yucatan Peninsula, Mexico. - Planta 242: 1425-1438, 2015. Go to original source...
    32. Souza C.S.C.R, Santos V.A.H.F., Ferreira M.J., Gonçalves J.F.C.: [Biomass, growth and ecophysiological responses of young plants of Bertholletia excelsa Bonpl. subjected to different levels of irradiance.] - Cienc. Florest. 27: 557-569, 2017. [In Portuguese] Go to original source...
    33. Tüffers A.V., Naid G., von Willert D.J.: The contribution of leaf angle to photoprotection in the mangroves Avicennia marina (FORSSK.) VIERH. and Bruguiera gymnorrhiza (L.) LAM. under field conditions in South Africa. - Flora 194: 267-275, 1999. Go to original source...
    34. Vaid S., Sharma S., Bajaj B.K.: Chemo-enzymatic approaches for consolidated bioconversion of Saccharum spontaneum biomass to ethanol-biofuel. - Bioresource Technol. 329: 124898, 2021. Go to original source...
    35. Wang C.W., Wong S.L., Liao T.S. et al.: Photosynthesis in response to salinity and submergence in two Rhizophoraceae mangroves adapted to different tidal elevations. - Tree Physiol. 42: 1016-1028, 2022. Go to original source...
    36. Weng J.H., Chen L.F., Jiang C.Y. et al.: A comparison between yellow-green and green cultivars of four vegetable species in pigments, ascorbate, photosynthesis, energy dissipation, and photoinhibition. - Photosynthetica 49: 361-370, 2011. Go to original source...
    37. Wong S.L., Chen C.W., Huang H.W., Weng J.H.: Using combined measurements of gas exchange and chlorophyll fluorescence to investigate the photosynthetic light responses of plant species adapted to different light regimes. - Photosynthetica 50: 206-214, 2012. Go to original source...
    38. Wu T.Y., Chen C.C., Lay H.L.: Study on the components and antioxidant activity of the Bletilla plant in Taiwan. - J. Food Drug Anal. 18: 279-289, 2010. Go to original source...
    39. Zulfugarov I.S., Ham O.K., Mishra S.R. et al.: Dependence of reaction center-type energy-dependent quenching on photosystem II antenna size. - BBA-Bioenergetics 1767: 773-780, 2007. Go to original source...

    Return to the content