Photosynthetica 2023, 61(1):1-12 | DOI: 10.32615/ps.2023.001

Diurnal changes in the stomatal, mesophyll, and biochemical limitations of photosynthesis in well-watered greenhouse-grown strawberries

G. YOKOYAMA1, †, S. ONO2, 3, †, D. YASUTAKE1, 4, K. HIDAKA5, T. HIROTA1
1 Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan
2 Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan
3 Research Fellow of the Japan Society for the Promotion of Science
4 IoP Collaborative Creation Center, Kochi University, B200 Monobe, Nankoku, 783-8502 Kochi, Japan
5 Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Kurume, 839-8503 Fukuoka, Japan

The diurnal variations in the factors of photosynthesis reduction under well-watered greenhouse conditions remain poorly understood. We conducted diurnal measurements of gas exchange and chlorophyll fluorescence in strawberries (Fragaria × ananassa Duch.) for three sunny days. Quantitative limitation analysis was also conducted to investigate the diurnal variations of photosynthetic limitations [stomatal (SL), mesophyll (MCL), and biochemical limitation (BL)]. Under well-watered greenhouse conditions, a photosynthesis reduction was observed, and the respective limitations exhibited different diurnal changes based on the environmental stress severity. The main limitation was SL, varying between 11.3 and 27.1% around midday, whereas MCL and BL were in 4.3-14.2% and 1.7-8.5%, respectively, under relatively moderate conditions. However, both SL (11.2-34.2%) and MCL (4.8-26.4%) predominantly limited photosynthesis under relatively severe conditions, suggesting that stomatal closure was the main limitation and that the decline in mesophyll conductance was not negligible under strong environmental stress, even under well-watered greenhouse conditions.

Additional key words: Fragaria × ananassa; midday depression; nonstomatal limitation; protected horticulture; stomatal limitation.

Received: December 5, 2022; Revised: December 5, 2022; Accepted: January 2, 2023; Prepublished online: January 25, 2023; Published: April 13, 2023  Show citation

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YOKOYAMA, G., ONO, S., YASUTAKE, D., HIDAKA, K., & HIROTA, T. (2023). Diurnal changes in the stomatal, mesophyll, and biochemical limitations of photosynthesis in well-watered greenhouse-grown strawberries. Photosynthetica, 61(1), 1-12. doi: 10.32615/ps.2023.001
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