Photosynthetica 2001, 39(1):29-33 | DOI: 10.1023/A:1012431516297

Gas Exchange of In Vitro and Ex Vitro Grown Grapevine Plants

T. Slavtcheva1, V. Dimitrova1
1 Institute of Viticulture and Enology, Pleven, Bulgaria

Net photosynthetic rate (PN) and dark respiration rate (RD) were measured in Vitis vinifera L. cvs. Dimiat 4/24 (23rd subculture), Dimiat 4/38 (22nd subculture), and Italian Riesling 3/47 (22nd subculture) on days 3, 2, and 1 (1st series) before transfer from the in vitro culture and on days 14, 15, 16 (2nd series) and 28, 29, 30 (3rd series) after the transfer. PN of in vitro and ex vitro plants was strongly affected by irradiance. PN and RD of in vitro plantlets were lower and transpiration rate (E) was higher compared to those of ex vitro plantlets. PN, RD, and E changed in the course of acclimation.

Additional key words: acclimation; dark respiration rate; fresh mass; leaf area; net photosynthetic rate; shoot and root lengths; shoot node number; transpiration rate; Vitis vinifera L.

Published: March 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Slavtcheva, T., & Dimitrova, V. (2001). Gas Exchange of In Vitro and Ex Vitro Grown Grapevine Plants. Photosynthetica, 39(1), 29-33. doi: 10.1023/A:1012431516297
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Barov, V., Naidenova, P.: [Statistical Methods for Field and Vegetative Experiments.]-Zemizdat, Sofia 1969. [In Bulg.]
    2. Bravdo, B., Lavee, S., Samish, R.M.: Analysis of water consumption of various grapevine cultivars-Vitis 10: 279-291, 1972.
    3. Dimitrova, V.: Clonal micropropagation of cv. Pamid (Vitis vinifera L., ssp. saliva) and nutrient medium composition optimization.-Wein-Wissenschaft 53: 168-171, 1998.
    4. Falque, M., Compan, D., Galzy, R.: A method for the study of CO2 exchange in in vitro cultured Vitis rupestris plantlets.-Plant Cell Tissue Organ Cult. 27: 175-181, 1991. Go to original source...
    5. Galzy, R., Compan, D.: Remarks on mixotrophic and autotrophic carbon nutrition of Vitis plantlets cultured in vitro.-Plant Cell Tissue Organ Cult. 31: 239-244, 1992. Go to original source...
    6. Galzy, R., Haffher, V., Compan, D.: Influence of three factors on the growth and nutrition of grapevine microcuttings.-J. exp. Bot. 41: 295-301, 1990. Go to original source...
    7. Geisler, G.: Art-und sortenspezifische CO2-Assimilationsraten von Reben unter Berücksichtigung wechselnder Beleuchtungsstärken.-Mitt. Klosterneuburg A 13: 301-305, 1963.
    8. Genoud, C., Coudret, A., Amalric, C., Sallanon, H.: Effects of micropropagation conditions of rose shootlets on chlorophyll fluorescence.-Photosynthetica 36: 243-251, 1999. Go to original source...
    9. Gribaudo, I., Fronda, A.: [Acclimation of micropropagated fruit plants.]-Riv. Frutt. 55: 75-80, 1993. [In Ital.]
    10. Iacono, F., De Micheli, L., Campostrini, F., Falcetti, M., Martinelli, L.: Comparison between photosynthesis and transpiration of in vitro cultured plantlets and in vivo cultured plants of genus Vitis. Implications of transplanting.-In: Gay, G., Gribaudo, L, Novello, V., Schubert, A. (ed.): Proc. 4th Int. Symp. Grapevine Physiol. Pp. 507-511. Giovanni Dalmasso Found., Turin 1993.
    11. Lakso, A.N., Reisch, B.I., Mortensen, J., Roberts, M.H.: Carbon dioxide enrichment for stimulation of growth of in vitro-propagated grapevines after transfer from culture.-J. amer. Soc. hort. Sci. 111: 634-638, 1986. Go to original source...
    12. Lee, N., Wetzstein, H.Y., Sommer, H.E.: Effects of quantum lux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival.-Plant Physiol. 78: 637-641, 1985. Go to original source...
    13. Lewandowski, V.T.: Rooting and acclimatization of micropropagated Vitis labruska "Dalaware".-Hort. Sci. 26: 586-589, 1991. Go to original source...
    14. Lima da Silva, A., Hariscain, P., Ollat, N., Doazan, J.P.: Estimation de la capacité photoautotrophique de vitroplants de porte-grefte de vigne (var. Gravesac): mise au point d'un systeme de mesure de la photosynthèse nette de vitroplants.-Vitis 35: 73-78, 1996.
    15. Pospíšilová, J., Čatský, J., Šesták, Z.: Photosynthesis in plants cultivated in vitro.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525-540. M. Dekker, New York-Basel-Hong Kong 1997.
    16. Pospíšilová, J., Tichá, I., Kadleček, P., Haisel, D., Plzáková, S.: Acclimatization of micropropagated plants to ex vitro conditions.-Biol. Plant. 42: 481-497, 1999. Go to original source...
    17. Schoch, P.-G., Lefèvre, B., Teisson, C., Ganry, J.: Photosynthèse et respiration de bananier in vitro.-Photosynthetica 23: 113-118, 1989.
    18. Slavtcheva, T., Dimitrova, V.: Gas exchange with in vitro cultured vine plants during their acclimatization.-Riv. Vitic. Enol. 50: 3-9, 1997.
    19. Slavtcheva, T., Dimitrova, V.: Rates of net photosynthesis and dark respiration of in vitro vine plants during acclimatization.-Vitis 38: 183-184, 1999.
    20. Solárová, J., Pospíšilová, J., Čatský, J., Šantrůček, J.: Photosynthesis and growth of tobacco plantlets in dependence on carbon supply.-Photosynthetica 23: 629-637, 1989.
    21. Stoev, K., Slavtcheva, T.: La photosynthèse nette chez la Vigne (V. vinifera L.) et les facteurs ecologiques.-Connais. Vigne Vin 16: 171-185, 1982. Go to original source...
    22. Vignes, D., Monange, Y., Carles, J.: Morphologic foliare et échanges gazeux chez la Vigne vierge.-Compt. rend. Acad. Sci. Paris D 278: 1365-1368, 1974.

    Return to the content