Photosynthetica 2002, 40(3):337-341 | DOI: 10.1023/A:1022610621721

Estimation of Photosynthetic Activity from the Electron Transport Rate of Photosystem 2 in a Film-Sealed Leaf of Sweet Potato, Ipomoea batatas Lam.

Haimeirong1, F. Kubota2, Y. Yoshimura1
1 Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, Japan
2 Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, Japan

In order to evaluate the photosynthetic activity of a C3 leaf from the electron transport rate (ETR) of photosystem 2 (PS2), a new method was devised and examined using leaves of sweet potato. In this method, both surfaces of a leaf were sealed with transparent films to stop the gas exchange between the leaf and the atmosphere; hence the functions of both photosynthetic assimilation (CO2 uptake) and photorespiration (CO2 release) are restricted to the inside of the leaf. After both functional rates became equally balanced, ETR of the sealed leaf (ETRseal) was determined from the chlorophyll fluorescence. The measurements were conducted at different irradiances and leaf temperatures and by using leaves of different age. Under each measurement condition, ETRseal showed a close positive relationship with the photosynthetic potential, or the gross photosynthetic rate measured in the air of 2 % O2 (PG2%) before sealing. ETRseal may become an indicator to estimate or evaluate the photosynthetic activity of C3 leaves.

Additional key words: C3-leaf; chlorophyll fluorescence; irradiance; leaf age; leaf-sealing method; leaf temperature; oxygen concentration; photorespiration; respiration

Published: September 1, 2002  Show citation

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Haimeirong,, Kubota, F., & Yoshimura, Y. (2002). Estimation of Photosynthetic Activity from the Electron Transport Rate of Photosystem 2 in a Film-Sealed Leaf of Sweet Potato, Ipomoea batatas Lam. Photosynthetica, 40(3), 337-341. doi: 10.1023/A:1022610621721
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