Photosynthetica 2007, 45(1):65-69 | DOI: 10.1007/s11099-007-0010-8

Effect of chromium on photosystem 2 in the unicellular green alga, Chlorella pyrenoidosa

Z. T. Hörcsik1, L. Kovács2, R. Láposi3, I. Mészáros3, G. Lakatos3, G. Garab2,*
1 Department of Zoology, College of Nyíregyháza, Nyíregyháza, Hungary
2 Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
3 Department of Botany, Debrecen University, Debrecen, Hungary

We investigated the effect of chromium (20-40 g m-3, 8-72 h) on the photosystem 2 (PS2) activities of Chlorella pyrenoidosa cells. By using chlorophyll fluorescence transients, thermoluminescence, oxygen polarography, and Western blot analysis for D1 protein we found that inhibition of PS2 can be accounted for by the enhanced photodestruction of the reaction centres in the cells cultivated in the presence of Cr(VI) at 25 °C in "white light" (18 W m-2). Hence photodestruction of D1 is caused by an enhanced oxidative stress and lipid peroxidation, as indicated by the appearance of a high-temperature thermoluminescence band.

Additional key words: chlorophyll fluorescence; D1 protein; Western blot analysis; thermoluminescence

Received: May 9, 2006; Accepted: June 26, 2006; Published: March 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hörcsik, Z.T., Kovács, L., Láposi, R., Mészáros, I., Lakatos, G., & Garab, G. (2007). Effect of chromium on photosystem 2 in the unicellular green alga, Chlorella pyrenoidosa. Photosynthetica, 45(1), 65-69. doi: 10.1007/s11099-007-0010-8
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Appenroth, K.J., Keresztes, A., Sárvári, É., Jaglarz, A., Fischer, W.: Multiple effects of chromate on Spirodela polyrhiza: Electron microscopy and biochemical investigations.-Plant Biol. 5: 315-323, 2003. Go to original source...
    2. Ball, J.W., Nordstrom, D.K.: Critical evaluation and selection of standard state thermodynamic properties for chromium metal and its aqueous ions, hydrolysis species, oxides, and hydroxides.-J. chem. Eng. Data 43: 895-918, 1998. Go to original source...
    3. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins.-Planta 170: 489-504, 1987. Go to original source...
    4. Clijsters, H., van Assche, F.: Inhibition of photosynthesis by heavy metals.-Photosynth. Res. 7: 31-40, 1985. Go to original source...
    5. Dixit, V., Pandey, V., Shyam, R.: Chromium ions inactivate electron transport and enhance superoxide generation in vivo in pea (Pisum sativum L. cv. Azad) root mitochondria.-Plant Cell Environ. 25: 687-693, 2002. Go to original source...
    6. Ducruet, J.M., Vavilin, D.: Chlorophyll high-temperature thermoluminescence emission as an indicator of oxidative stress: Perturbating effects of oxygen and leaf water content.-Free Radical Res. 31: S187-S192, 1999. Go to original source...
    7. Genty, B., Harbinson, J., Briantais, J.-M., Baker, N.R.: The relationship between nonphotochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves.-Photosynth. Res. 25: 249-257, 1990. Go to original source...
    8. Havaux, M.: Spontaneous and thermoinduced photon emission: new methods to detect and quantify oxidative stress in plants.-Trends Plant Sci. 8: 409-413, 2003. Go to original source...
    9. Kabata-Pendias, A., Pendias, H. Trace Elements in Soils and Plants. 3rd Ed.-CRC Press, Boca Raton-London-New York-Washington 2001. Go to original source...
    10. Krupa, Z., Baszyński, T.: Some aspects of heavy metals toxicity towards photosynthetic apparatus - direct and indirect effects on light and dark reactions.-Acta Physiol. Plant. 17: 177-190, 1995.
    11. Küpper, H., ©etlík, I., Spiller, M., Küpper, F.C., Práąil, O.: Heavy metal-induced inhibition of photosynthesis: Targets of in vivo heavy metal chlorophyll formation.-J. Phycol. 38: 429-441, 2002. Go to original source...
    12. Lustigman, B., Lee, L.H., Khalil, A.: Effects of nickel and pH on the growth of Chlorella vulgaris.-Bull. Environ. Contamin. Toxicol. 55: 73-80, 1995. Go to original source...
    13. Mizusawa, N., Tomo, T., Satoh, K., Miyao, M.: Degradation of the D1 protein of photosystem II under illumination in vivo: Two different pathways involving cleavage or intermolecular cross-linking.-Biochemistry 42: 10034-10044, 2003. Go to original source...
    14. Nash, S.M.B., Quayle, P.A., Schreiber, U., Muller, J.F.: The selection of a model microalgal species as biomaterial for a novel aquatic phytotoxicity assay.-Aquat. Toxicol. 72: 315-326, 2005. Go to original source...
    15. Niyogi, K.K.: Photoprotection revisited: Genetic and molecular approaches.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 333-359, 1999. Go to original source...
    16. Ohira, S., Morita, N., Suh, H.J., Jung, J., Yamamoto, Y.: Quality control of photosystem II under light stress - turnover of aggregates of the D1 protein in vivo.-Photosynth. Res. 84: 29-33, 2005. Go to original source...
    17. Rachlin, J.W., Grosso, A.: The growth response of the green alga Chlorella vulgaris to combined cation exposure.-Arch. Environ. Contamin. Toxicol. 24: 16-20, 1993. Go to original source...
    18. Rai, V., Vajpayee, P., Singh, S.N., Mehrotra, S.: Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L.-Plant Sci. 167: 1159-1169, 2004. Go to original source...
    19. Sandalio, L.M., Dalurzo, H.C., Gomez, M., Romero-Puertas, M.C., del Rio, L.A.: Cadmium-induced changes in the growth and oxidative metabolism of pea plants.-J. exp. Bot. 52: 2115-2126, 2001. Go to original source...
    20. Shanker, K.A., Cervantes, C., Loza-Tavera, H., Avudainayagam, S.: Chromium toxicity in plants.-Environ. int. 31: 739-753, 2005. Go to original source...
    21. Shriver, D.F., Atkins, P.W., Langford, C.H.: Inorganic Chemistry. 2nd Ed.-Oxford University Press, Oxford 1990.
    22. Somashekaraiah, B.V., Padmaja, K., Prasad, A.R.K.: Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation.-Physiol. Plant. 85: 85-89, 1992. Go to original source...
    23. Towbin, H., Stachelin, T., Gordon, J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.-Proc. nat. Acad. Sci. USA 76: 4350-4354, 1979. Go to original source...
    24. Wiessner, W., Demeter, S.: Comparative thermoluminescence study of autotrophically and photoheterotrophically cultivated Chlamydobotrys stellata.-Photosynth. Res. 18: 345-356, 1988. Go to original source...
    25. Zeid, I.M.: Responses of Phaseolus vulgaris to chromium and cobalt treatments.-Biol. Plant. 44: 111-115, 2001. Go to original source...
    26. Zsiros, O., Allakhverdiev, S.I., Hihashi, S., Watanabe, M., Nishiyama, Y., Murata, N.: Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair.-Biochim. biophys. Acta 1757: 123-129, 2006. Go to original source...

    Return to the content