Photosynthetica 2025, 63(1):37-45 | DOI: 10.32615/ps.2025.003

Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers

S. TANIOS, T. THANGAVEL, A. EYLES, R.S. TEGG, C.R. WILSON
Tasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, Australia

Potato tuber greening occurs due to the chlorophyll accumulation upon exposure to light, however, fundamental information on tuber chlorophyll metabolism is lacking. We measured the effect of varying light exposure (0, 48, 96, and 168 h) on chlorophyll concentration and gene expression of enzymes in the chlorophyll metabolic pathway in the potato varieties that differ in greening propensity. Greening was associated with the upregulation of genes involved in chlorophyll biosynthesis, particularly glutamyl-tRNA reductase 1, magnesium-chelatase subunit H, and magnesium-protoporphyrin IX monomethyl ester cyclase, and downregulation of genes involved in chlorophyll cycling and degradation, including chlorophyllide a oxygenase, and pheophorbide a oxygenase. Our findings suggest that relative resistance to tuber greening propensity may be due to a weaker upregulation of chlorophyll biosynthesis genes and weaker downregulation of chlorophyll degradation genes that occurs in susceptible varieties. The association of these biosynthesis and degradation genes with greening susceptibility may provide possible breeding targets for the future development of more greening-resistant varieties.

Additional key words: chlorophyll biosynthesis; chlorophyll degradation; chlorophyll metabolic pathway; tuber greening.

Received: October 2, 2024; Revised: January 10, 2025; Accepted: January 28, 2025; Prepublished online: February 13, 2025; Published: March 27, 2025  Show citation

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TANIOS, S., THANGAVEL, T., EYLES, A., TEGG, R.S., & WILSON, C.R. (2025). Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers. Photosynthetica, 63(1), 37-45. doi: 10.32615/ps.2025.003
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    References

    1. Alawady A.E., Grimm B.: Tobacco Mg protoporphyrin IX methyltransferase is involved in inverse activation of Mg porphyrin and protoheme synthesis. - Plant J. 41: 282-290, 2005. Go to original source...
    2. Anstis P.J.P., Northcote D.H.: Development of chloroplasts from amyloplasts in potato tuber discs. - New Phytol. 72: 449-463, 1973. Go to original source...
    3. Bamberg J., Moehninsi, Navarre R., Suriano J.: Variation for tuber greening in the diploid wild potato Solanum microdontum. - Am. J. Potato Res. 92: 435-443, 2015. Go to original source...
    4. Chen M.: Chlorophyll modifications and their spectral extension in oxygenic photosynthesis. - Annu. Rev. Biochem. 83: 317-340, 2014. Go to original source...
    5. Diretto G., Al-Babili S., Tavazza R. et al.: Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway. - PLoS ONE 2: e350, 2007. Go to original source...
    6. Espineda C.E., Linford A.S., Devine D., Brusslan J.A.: The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana. - PNAS 96: 10507-10511, 1999. Go to original source...
    7. Ha J., Moon K., Kim M. et al.: The laccase promoter of potato confers strong tuber-specific expression in transgenic plants. -Plant Cell Tiss. Org. Cult. 120: 57-68, 2015. Go to original source...
    8. Jung K.-H., Hur J., Ryu C.-H. et al.: Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. - Plant Cell Physiol. 44: 463-472, 2003. Go to original source...
    9. Kumar A.M., Csankovszki G., Söll D.: A second and differentially expressed glutamyl-tRNA reductase gene from Arabidopsis thaliana. - Plant Mol. Biol. 30: 419-426, 1996. Go to original source...
    10. Li M., Song B., Zhang Q. et al.: A synthetic tuber-specific and cold-induced promoter is applicable in controlling potato cold-induced sweetening. - Plant Physiol. Biochem. 67: 41-47, 2013. Go to original source...
    11. Liebers M., Grübler B., Chevalier F. et al.: Regulatory shifts in plastid transcription play a key role in morphological conversions of plastids during plant development. - Front. Plant Sci. 8: 23, 2017. Go to original source...
    12. Liu X., Li Y., Zhong S.: Interplay between light and plant hormones in the control of Arabidopsis seedling chlorophyll biosynthesis. - Front. Plant Sci. 8: 1433, 2017. Go to original source...
    13. Matsumoto F., Obayashi T., Sasaki-Sekimoto Y. et al.: Gene expression profiling of the tetrapyrrole metabolic pathway in Arabidopsis with a mini-array system. - Plant Physiol. 135: 2379-2391, 2004. Go to original source...
    14. McCormac A.C., Fischer A., Kumar A.M. et al.: Regulation of HEMA1 expression by phytochrome and a plastid signal during de-etiolation in Arabidopsis thaliana. - Plant J. 25: 549-561, 2001. Go to original source...
    15. McCormac A.C., Terry M.J.: Light-signalling pathways leading to the co-ordinated expression of HEMA1 and Lhcb during chloroplast development in Arabidopsis thaliana. - Plant J. 32: 549-559, 2002. Go to original source...
    16. Meinecke L., Alawady A., Schroda M. et al.: Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. - Plant Mol. Biol. 72: 643-658, 2010. Go to original source...
    17. Murashige R., Skoog F.: A revised medium for rapid growth and bio assays with tobacco tissue culture. - Physiol. Plantarum 15: 473-479, 1962. Go to original source...
    18. Nicot N., Hausman J.-F., Hoffmann L., Evers D.: Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. - J. Exp. Bot. 56: 2907-2914, 2005. Go to original source...
    19. Ohmiya A., Hirashima M., Yagi M. et al.: Identification of genes associated with chlorophyll accumulation in flower petals. - PLoS One 9: e113738, 2014. Go to original source...
    20. Ohmiya A., Sasaki K., Nashima K. et al.: Transcriptome analysis in petals and leaves of chrysanthemums with different chlorophyll levels. - BMC Plant Biol. 17: 202, 2017. Go to original source...
    21. Okamoto H., Ducreux L.J.M., Allwood J.W. et al.: Light regulation of chlorophyll and glycoalkaloid biosynthesis during tuber greening of potato S. tuberosum. - Front. Plant Sci. 11: 753, 2020. Go to original source...
    22. Porra R.J., Thompson W.A., Kriedemann P.E.: Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. - BBA-Bioenergetics 975: 384-394, 1989. Go to original source...
    23. Pru¾inská A., Tanner G., Aubry S. et al.: Chlorophyll breakdown in senescent Arabidopsis leaves. Characterization of chlorophyll catabolites and of chlorophyll catabolic enzymes involved in the degreening reaction. - Plant Physiol. 139: 52-63, 2005. Go to original source...
    24. Stephenson P.G., Terry M.J.: Light signalling pathways regulating the Mg-chelatase branchpoint of chlorophyll synthesis during de-etiolation in Arabidopsis thaliana. - Photoch. Photobio. Sci. 7: 1243-1252, 2008. Go to original source...
    25. Su Q., Frick G., Armstrong G., Apel K.: POR C of Arabidopsis thaliana: a third light- and NADPH-dependent proto-chlorophyllide oxidoreductase that is differentially regulated by light. - Plant Mol. Biol. 47: 805-813, 2001. Go to original source...
    26. Tanaka R., Tanaka A.: Tetrapyrrole biosynthesis in higher plants. - Annu. Rev. Plant Biol. 58: 321-346, 2007. Go to original source...
    27. Tanios S., Eyles A., Corkrey R. et al.: Quantifying risk factors associated with light-induced potato tuber greening in retail stores. - PLoS ONE 15: e0235522, 2020a. Go to original source...
    28. Tanios S., Eyles A., Tegg R.S., Wilson C.: Potato tuber greening: a review of predisposing factors, management and future challenges. - Am. J. Potato Res. 95: 248-257, 2018. Go to original source...
    29. Tanios S., Tegg R.S., Eyles A. et al.: Potato tuber greening risk is associated with tuber nitrogen content. - Am. J. Potato Res. 97: 360-366, 2020b. Go to original source...
    30. Tanios S., Thangavel T., Eyles A. et al.: Suberin deposition in potato periderm: a novel resistance mechanism against tuber greening. - New Phytol. 225: 1273-1284, 2020c. Go to original source...
    31. Ujwal M.L., McCormac A.C., Goulding A. et al.: Divergent regulation of the HEMA gene family encoding glutamyl-tRNA reductase in Arabidopsis thaliana: expression of HEMA2 is regulated by sugars, but is independent of light and plastid signalling. - Plant Mol. Biol. 50: 81-89, 2002. Go to original source...
    32. Wang J., Li J., Chen W. et al.: The changes in chlorophyll, solanine, and phytohormones during light-induced greening in postharvest potatoes. - Postharvest Biol. Tec. 219: 113291, 2025. Go to original source...
    33. Wang X., Huang R., Quan R.: Mutation in Mg-protoporphyrin IX monomethyl ester cyclase decreases photosynthesis capacity in rice. - PLoS ONE 12: e0171118, 2017. Go to original source...
    34. Yuan M., Zhao Y.-Q., Zhang Z.-W. et al.: Light regulates transcription of chlorophyll biosynthetic genes during chloroplast biogenesis. - Crit. Rev. Plant Sci. 36: 35-54, 2017. Go to original source...
    35. Zhu Y.S., Merkle-Lehman D.L., Kung S.D.: Light-induced transformation of amyloplasts into chloroplasts in potato tubers. - Plant Physiol. 75: 142-145, 1984. Go to original source...

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